What can we learn from x-ray diffuse scatter from protein crystals?

Abstract:

Conformational changes drive protein function, including catalysis, allostery and signaling. X-ray diffuse scattering from protein crystals has frequently been cited as a probe of these correlated motions, with significant potential to advance our understanding of biological dynamics. However, recent work has challenged this prevailing view, suggesting instead that diffuse scattering primarily originates from rigid-body motions and could therefore be applied to improve structure determination. We have systematically investigated the physical origins of diffuse scatter from protein crystals. I will present our findings and a perspective for the future of this exciting topic.