Light Sheet Microscopy Pilot Facility

Light Sheet Fluorescence Microscopy permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution. The facility offers access to a commercially available Lightsheet Z.1 (Zeiss) microscope, allowing users to image live and optically cleared samples at unprecedented speeds. Collaborative project support is provided with sample mounting, image acquisitions, post-acquisition processing and analysis of the resulting data.

Services

  • Light Sheet microscopy - live, fixed and optically cleared samples
  • Sample preparation and mounting
  • Post-acquisition processing - Image stitching, multiview fusion and deconvolution
  • Data analysis - Arivis Vision 4D and Imaris 8.x

Equipment

Zeiss Light Sheet Z.1 including:

  • Dual side Illumination with pivot scan to reduce shadows and striping artifacts
  • CO2 and temperature control modules
  • Two sCMOS PCOedge cameras for simultanous dual colour acquisition

Objectives:

  • 5x/0.16 air objective
  • 10x/0.5, 20x/1.0 and 40x/1.0 water dipping objectives
  • 20x/1.0 clearing objective (RI 1.42 - 1.48) - FocusClear, TDE, Histodenz/Omnipaque etc.


Laser lines:

  • 405, 488, 561, 638nm

Filter cubes:

  • BP 505-545 + BP 575-615
  • BP 505-545 + LP 585
  • SP 550 + LP 585
  • BP 505 - 530 + LP 585
  • BP 420 - 470 + BP 505 - 545

Applications

  • Morphogenesis, Embryogenesis and Organogenesis - typically in model organisms such as zebrafish and Drosophila melanogaster
  • 3D cell culture - typically spheroids, tissue and organotypic culture
  • Imaging optically cleared samples - CLARITY, PARS, iDISCO etc.
  • Expansion microscopy
  • Plants - Arabidopsis

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The Light Sheet facility is hosted by SciLifeLab.

Contact

Steven Edwards

Pilot Lightsheet Facility Head

Email: steven.edwards@scilifelab.se

Using the facility

If you are interested in using the facility, please fill out the access form found here:

Access-form to Light sheet facility (docx 17 kB)

Visiting address

SciLifeLab, Gamma 3
Tomtebodavägen 23
171 21 Solna
Sweden

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