Soft x-ray microscopy
Senior scientist(s): Hans Hertz, Jonas Sellberg
Microscopy in the water window (=2.3-4.4 nm; E=284-540 eV) allows for imaging of unstained intact cells in their near-native environment with unprecedented resolution and contrast . Synchrotron-based x-ray microscopy has demonstrated significant progress the last few years, delivering results of high biological relevance with second-range exposure times. Laboratory microscopy now produces images with synchrotron quality but with longer exposure times.
We demonstrated the first sub-visible-resolution laboratory water-window x-ray microscope . The microscope relies on our pioneering work on high-brightness liquid-jet laser-plasma sources , normal-incidence multilayer condenser optics, and in-house diffractive optics. Recent work include 3D cryo-tomography of intact cells (see Figure 1) and imaging of giant DNA virus [4,5]. The resolution is down to 25 nm and 10 s exposure times were recently demonstrated .
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