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Dr. Anna-Karin Gustavsson - Institutionen för Fysik, Göteborgs Universitet

Glycolytic oscillations in individual yeast cells

Time: Thu 2015-03-12 09.00 - 10.11

Location: FA32

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Titel: Glycolytic oscillations in individual yeast cells

Abstract:

Oscillations in the concentration of yeast glycolytic intermediates have been intensively studied since the 1950s, but these studies have so far been limited to observations of average oscillatory behavior in synchronized cultures. To determine the mechanisms behind oscillations, cell-cell interactions and synchronization, and to investigate the role of cell-cell heterogeneity, oscillations have to be studied on the single-cell level.

At this seminar I will present how glycolytic oscillations can be induced and studied in individual, isolated yeast cells by combining optical tweezers for cell positioning, microfluidics for environmental control and fluorescence microscopy for detection.

The results from these studies have advanced our understanding of the complex set of reactions in energy metabolism and the mechanisms through which cells oscillate, communicate, and synchronize. Pursuing these studies will hopefully not only give further information about glycolysis in yeast, but also about energy metabolism, oscillations, and communication in other biological systems, such as oscillatory insulin secretion from islets of β-cells.

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Belongs to: Department of Applied Physics
Last changed: Nov 26, 2015