Cell physics

Our laboratory is a multidisciplinary combination of the Cell Physics research group from KTH and the Pediatric Cell and Molecular Biology group from KI. The laboratory is located in the Science for Life Laboratory. We use an interdisciplinary and translational approach to address questions of high medical relevance. Our biomedical emphasis is on the molecular origin for some pediatric and neuropsychiatric diseases. Our biophysical emphasis is on cellular biophysics, advanced light microscopy and nanobiophotonics - nanoscopy.

National Research Facility - Superresolution Microscopy

The Advanced Light Microscopy (ALM) facility give open-access to state-of-the art superresolution fluorescence microscopy for nanoscale biological visualization - nanoscopy. The facility offers access to super-resolution techniques (STED, PALM/STORM, SIM) and to experimental techniques such as easy-STED and QD-SPT. For more information and access see  www.scilifelab.se/facilities/alm.

All our systems are now upgraded to 3D functionality (3D-STED, 3D-PALM, 3D-SIM)

BRISMAR GROUP

The main topic in our research is to understand the many functional aspects of Na+,K+-ATPase, the salt pump that is the major determinant of intracellular ion homeostasis and which has recently been shown to also function as a signal transducer.

Studies of Na+,K+ –ATPase are important, since a number of monogenetic diseases, associated with cognitive deficits, therapy resistant epilepsy and dystonia, have recently been found to be due to single mutations in the catalytic subunit of the neuronal isoform of Na+,K+-ATPase. We have made several pioneering contributions to the understanding of the specific function of neuronal Na+,K+-ATPase, by identification of its subcellular localization in the neuron with STED and PALM microscopy and by demonstrating its functional importance for recovery of intracellular sodium after high neuronal activity with subcellular real time sodium imaging. Currently we are studying the functional consequences of disease mutations, using a variety of imaging and modeling approaches.

We have also pioneered the field of Na+,K+-ATPase signaling, describing how the endogenous cardiotonic steroid ouabain, which is a specific Na+,K+-ATPase ligand, triggers an oscillating calcium signal, where the downstream effects are activation of the anti-apoptotic factor Bcl-xL and protection from apoptosis. This signal has been shown to play a major role for pattern formation during embryonic development and we are now seeking to identify the genes activated by the ouabain/Na+,K+-ATPase calcium signal.

ÖNFELT GROUP - Cell Screening and Migration

Our research focus is on the immune system and in particular on NK and T cells. The lab is multidisciplinary and combines biology and technology. One of the immediate goals of our research is to implement new tools and single cell assays that can be used in academic research, hospitals or in pharmacological industry for immune cell diagnostics, applications within stem cell transplantation and cellular therapy.

FU GROUP - Quantum dots development

Quantum dots, when you want to see more for a longer time... The use of colloidal quantum dots (QDs) is one of the most exciting developments in nanobiotechnology. Because of their high durability and unique optical properties QDs are widely used as fluorescent labelling agents for in vitro and in vivo bioimaging, such as cell labeling, deep tissue imaging, and fluorescent resonance energy transfer donors. Surface modified and water-soluble QDs open a new era in cell imaging and biotargeting.

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